A murine model for assessment of living attenuated influenza A vaccines.
Identifieur interne : 002786 ( Main/Exploration ); précédent : 002785; suivant : 002787A murine model for assessment of living attenuated influenza A vaccines.
Auteurs : M C Wark ; G A Tannock ; M M Sutherland ; L E SmithSource :
- The Australian journal of experimental biology and medical science [ 0004-945X ] ; 1980.
Descripteurs français
- KwdFr :
- MESH :
- croissance et développement : Virus de la grippe A.
- génétique : Virus de la grippe A.
- microbiologie : Poumon.
- Animaux, Modèles biologiques, Mutation, Mâle, Souris, Température, Vaccins antigrippaux, Vaccins atténués.
English descriptors
- KwdEn :
- MESH :
- chemical : Influenza Vaccines, Vaccines, Attenuated.
- genetics : Influenza A virus.
- growth & development : Influenza A virus.
- microbiology : Lung.
- Animals, Male, Mice, Models, Biological, Mutation, Temperature.
Abstract
The laboratory mouse was evaluated as a model to assess the genetic stability of influenza A mutants of potential use as living vaccine strains. The growth of three mutant recombinants, A/Hong Kong/68-ts-1E (H3N2), A/HK/123/77x-ts-1A2 (H1N1) and A2/AA/6/60-ca(H2N2) was studied in 15 g mice. Yields of ts-1E from both lungs and turbinates were ten-fold less than that of a control virus with the same surface antigens. All ts-1E isolates showed evidence of loss of ts phenotype. Ts-1A2 and ca recombinants grew to a much lower titre than those of ts-1E, and revertants were obtained from one ts-1A2 lung isolate and one ca turbinate isolate. In other studies with hamsters, the stability of the ts character of these mutants during replication in the lungs of hamsters has been shown to be correlated with their residual virulence for man (Murphy et al., 1972; Murphy et al., 1974; Richman et al., 1977). The results from the present study suggest that the laboratory mouse is at least as sensitive as the hamster as an in vitro model for the detection of ts revertants.
DOI: 10.1038/icb.1980.64
PubMed: 7271601
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000587
- to stream PubMed, to step Curation: 000587
- to stream PubMed, to step Checkpoint: 000519
- to stream Ncbi, to step Merge: 001267
- to stream Ncbi, to step Curation: 001267
- to stream Ncbi, to step Checkpoint: 001267
- to stream Main, to step Merge: 002933
- to stream Main, to step Curation: 002786
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A murine model for assessment of living attenuated influenza A vaccines.</title><author><name sortKey="Wark, M C" sort="Wark, M C" uniqKey="Wark M" first="M C" last="Wark">M C Wark</name></author><author><name sortKey="Tannock, G A" sort="Tannock, G A" uniqKey="Tannock G" first="G A" last="Tannock">G A Tannock</name></author><author><name sortKey="Sutherland, M M" sort="Sutherland, M M" uniqKey="Sutherland M" first="M M" last="Sutherland">M M Sutherland</name></author><author><name sortKey="Smith, L E" sort="Smith, L E" uniqKey="Smith L" first="L E" last="Smith">L E Smith</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1980">1980</date><idno type="RBID">pubmed:7271601</idno><idno type="pmid">7271601</idno><idno type="doi">10.1038/icb.1980.64</idno><idno type="wicri:Area/PubMed/Corpus">000587</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000587</idno><idno type="wicri:Area/PubMed/Curation">000587</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000587</idno><idno type="wicri:Area/PubMed/Checkpoint">000519</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000519</idno><idno type="wicri:Area/Ncbi/Merge">001267</idno><idno type="wicri:Area/Ncbi/Curation">001267</idno><idno type="wicri:Area/Ncbi/Checkpoint">001267</idno><idno type="wicri:doubleKey">0004-945X:1980:Wark M:a:murine:model</idno><idno type="wicri:Area/Main/Merge">002933</idno><idno type="wicri:Area/Main/Curation">002786</idno><idno type="wicri:Area/Main/Exploration">002786</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A murine model for assessment of living attenuated influenza A vaccines.</title><author><name sortKey="Wark, M C" sort="Wark, M C" uniqKey="Wark M" first="M C" last="Wark">M C Wark</name></author><author><name sortKey="Tannock, G A" sort="Tannock, G A" uniqKey="Tannock G" first="G A" last="Tannock">G A Tannock</name></author><author><name sortKey="Sutherland, M M" sort="Sutherland, M M" uniqKey="Sutherland M" first="M M" last="Sutherland">M M Sutherland</name></author><author><name sortKey="Smith, L E" sort="Smith, L E" uniqKey="Smith L" first="L E" last="Smith">L E Smith</name></author></analytic><series><title level="j">The Australian journal of experimental biology and medical science</title><idno type="ISSN">0004-945X</idno><imprint><date when="1980" type="published">1980</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Influenza A virus (genetics)</term><term>Influenza A virus (growth & development)</term><term>Influenza Vaccines</term><term>Lung (microbiology)</term><term>Male</term><term>Mice</term><term>Models, Biological</term><term>Mutation</term><term>Temperature</term><term>Vaccines, Attenuated</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Modèles biologiques</term><term>Mutation</term><term>Mâle</term><term>Poumon (microbiologie)</term><term>Souris</term><term>Température</term><term>Vaccins antigrippaux</term><term>Vaccins atténués</term><term>Virus de la grippe A (croissance et développement)</term><term>Virus de la grippe A (génétique)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Influenza Vaccines</term><term>Vaccines, Attenuated</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Male</term><term>Mice</term><term>Models, Biological</term><term>Mutation</term><term>Temperature</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Modèles biologiques</term><term>Mutation</term><term>Mâle</term><term>Souris</term><term>Température</term><term>Vaccins antigrippaux</term><term>Vaccins atténués</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The laboratory mouse was evaluated as a model to assess the genetic stability of influenza A mutants of potential use as living vaccine strains. The growth of three mutant recombinants, A/Hong Kong/68-ts-1E (H3N2), A/HK/123/77x-ts-1A2 (H1N1) and A2/AA/6/60-ca(H2N2) was studied in 15 g mice. Yields of ts-1E from both lungs and turbinates were ten-fold less than that of a control virus with the same surface antigens. All ts-1E isolates showed evidence of loss of ts phenotype. Ts-1A2 and ca recombinants grew to a much lower titre than those of ts-1E, and revertants were obtained from one ts-1A2 lung isolate and one ca turbinate isolate. In other studies with hamsters, the stability of the ts character of these mutants during replication in the lungs of hamsters has been shown to be correlated with their residual virulence for man (Murphy et al., 1972; Murphy et al., 1974; Richman et al., 1977). The results from the present study suggest that the laboratory mouse is at least as sensitive as the hamster as an in vitro model for the detection of ts revertants.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Smith, L E" sort="Smith, L E" uniqKey="Smith L" first="L E" last="Smith">L E Smith</name><name sortKey="Sutherland, M M" sort="Sutherland, M M" uniqKey="Sutherland M" first="M M" last="Sutherland">M M Sutherland</name><name sortKey="Tannock, G A" sort="Tannock, G A" uniqKey="Tannock G" first="G A" last="Tannock">G A Tannock</name><name sortKey="Wark, M C" sort="Wark, M C" uniqKey="Wark M" first="M C" last="Wark">M C Wark</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002786 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002786 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:7271601
|texte= A murine model for assessment of living attenuated influenza A vaccines.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:7271601" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a H2N2V1
| This area was generated with Dilib version V0.6.33. |  |